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The effect of dopamine D(2) receptor antagonists and agonists on adenosine 3',5'-cyclic monophosphate (cAMP) formation in the rat striatum was studied in vitro. The antagonists, haloperidol and sulpiride, inhibited forskolin-stimulated cAMP formation in a concentration-dependent manner, with IC(50) values of 10.3 and 39.0 nM, respectively. The D(2) receptor antagonists, sulpiride and (-)-sulpiride, inhibited forskolin-stimulated cAMP formation in a concentration-dependent manner, with IC(50) values of 5.1 and 3.6 nM, respectively, whereas the D(2) receptor antagonists, domperidone, nomifensine, and spiperone, were less effective in inhibiting forskolin-stimulated cAMP formation. The D(2) receptor agonists, quinpirole and N-0923, stimulated cAMP formation with EC(50) values of 0.4 and 0.8 nM, respectively.
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